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16S rRNA Terminal Restriction Fragment Length Polymorphism for the Characterization of the Nasopharyngeal Microbiota

A novel non-culture based 16S rRNA Terminal Restriction Fragment Length Polymorphism (T-RFLP) method using the restriction enzymes Tsp509I and Hpy166II was developed for the characterization of the nasopharyngeal microbiota and validated using recently published 454 pyrosequencing data. 16S rRNA gen...

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Main Authors: Brugger, Silvio D., Frei, Laurence, Frey, Pascal M., Aebi, Suzanne, Mühlemann, Kathrin, Hilty, Markus
格式: Artigo
語言:Inglês
出版: Public Library of Science 2012
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3527403/
https://ncbi.nlm.nih.gov/pubmed/23284951
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0052241
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