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Effective flow cytometric phenotyping of cells using minimal amounts of antibody

Here we introduce a modified antibody staining method that uses up to 80% less antibody for flow cytometry. We demonstrate this method for the detection of antigens expressed at high, moderate, or low levels in mouse and rat lymphocytes as well as mouse mammary epithelial cells. We obtained reproduc...

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Autors principals: Sharma, Deepak, Eichelberg, Mark R., Haag, Jill D, Meilahn, Amanda L., Muelbl, Matthew J., Schell, Kathy, Smits, Bart M. G., Gould, Michael N.
Format: Artigo
Idioma:Inglês
Publicat: 2012
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3523330/
https://ncbi.nlm.nih.gov/pubmed/22780320
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/0000113854
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