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Enzymatic Amplification of DNA/RNA Hybrid Molecular Beacon Signaling in Nucleic Acid Detection

A rapid assay operable under isothermal or non-isothermal conditions is described wherein the sensitivity of a typical molecular beacon (MB) system is improved by utilizing thermostable RNase H to enzymatically cleave an MB comprised of a DNA stem and RNA loop (R/D-MB). Upon hybridization of the R/D...

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Détails bibliographiques
Auteurs principaux: Jacroux, Thomas, Rieck, Daniel C., Cui, Rong, Ouyang, Yexin, Dong, Wen-Ji
Format: Artigo
Langue:Inglês
Publié: 2012
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3522425/
https://ncbi.nlm.nih.gov/pubmed/23000602
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.09.015
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