Human IgA-binding Peptides Selected from Random Peptide Libraries: AFFINITY MATURATION AND APPLICATION IN IGA PURIFICATION

Phage display system is a powerful tool to design specific ligands for target molecules. Here, we used disulfide-constrained random peptide libraries constructed with the T7 phage display system to isolate peptides specific to human IgA. The binding clones (A1–A4) isolated by biopanning exhibited cl...

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Detalhes bibliográficos
Main Authors: Hatanaka, Takaaki, Ohzono, Shinji, Park, Mirae, Sakamoto, Kotaro, Tsukamoto, Shogo, Sugita, Ryohei, Ishitobi, Hiroyuki, Mori, Toshiyuki, Ito, Osamu, Sorajo, Koichi, Sugimura, Kazuhisa, Ham, Sihyun, Ito, Yuji
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2012
Assuntos:
Protein Structure and Folding
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3522307/
https://ncbi.nlm.nih.gov/pubmed/23076147
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M112.389742
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