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Generation of a Genome Scale Lentiviral Vector Library for EF1α Promoter-Driven Expression of Human ORFs and Identification of Human Genes Affecting Viral Titer

The bottleneck in elucidating gene function through high-throughput gain-of-function genome screening is the limited availability of comprehensive libraries for gene overexpression. Lentiviral vectors are the most versatile and widely used vehicles for gene expression in mammalian cells. Lentiviral...

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Hlavní autoři: Škalamera, Dubravka, Dahmer, Mareike, Purdon, Amy S., Wilson, Benjamin M., Ranall, Max V., Blumenthal, Antje, Gabrielli, Brian, Gonda, Thomas J.
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3520899/
https://ncbi.nlm.nih.gov/pubmed/23251614
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0051733
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