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Activation of Alpha Chymotrypsin by Three Phase Partitioning Is Accompanied by Aggregation

Precipitation of alpha chymotrypsin in the simultaneous presence of ammonium sulphate and t-butanol (three phase partitioning) resulted in preparations which showed self aggregation of the enzyme molecules. Precipitation with increasing amounts of ammonium sulphate led to increasing size of aggregat...

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Detalhes bibliográficos
Main Authors: Rather, Gulam Mohmad, Mukherjee, Joyeeta, Halling, Peter James, Gupta, Munishwar Nath
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3519768/
https://ncbi.nlm.nih.gov/pubmed/23239966
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0049241
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