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A Pre-Steady State Kinetic Analysis of the αY60W mutant of trans-3-Chloroacrylic Acid Dehalogenase: Implications for the Mechanism of the Wild-type Enzyme()

The bacterial degradation of the nematicide 1,3-dichloropropene, an isomeric mixture, requires the action of trans- and cis-3-chloracrylic acid dehalogenase (CaaD and cis-CaaD, respectively). Both enzymes are tautomerase superfamily members and share a core catalytic mechanism for the hydrolytic deh...

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Detalhes bibliográficos
Main Authors: Huddleston, Jamison P., Schroeder, Gottfried K., Johnson, Kenneth A., Whitman, Christian P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3518313/
https://ncbi.nlm.nih.gov/pubmed/23110338
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi3010686
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