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Genetically Encoded Cyclopropene Directs Rapid, Photoclick Chemistry-Mediated Protein Labeling in Mammalian Cells
Genetic incorporation of a cyclopropene amino acid, N(ε)-(1-methylcycloprop-2-enecarboxamido)-lysine (CpK), into sperm whale myoglobin site-specifically in E. coli as well as enhanced green fluorescent protein in mammalian cells was achieved through amber codon suppression employing an orthogonal am...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3517012/ https://ncbi.nlm.nih.gov/pubmed/22997015 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201205352 |
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