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Agarose Gel Electrophoresis Reveals Structural Fluidity of a Phage T3 DNA Packaging Intermediate

We find a new aspect of DNA packaging-associated structural fluidity for phage T3 capsids. The procedure is (1) glutaraldehyde cross-linking of in vivo DNA packaging intermediates for stabilization of structure and then (2) determining of effective radius by two-dimensional agarose gel electrophores...

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Bibliographic Details
Main Authors: Serwer, Philip, Wright, Elena T.
Format: Artigo
Language:Inglês
Published: 2012
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3516883/
https://ncbi.nlm.nih.gov/pubmed/22222979
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.201100326
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