Quantification of Reverse Transcriptase Activity by Real-Time PCR as a Fast and Accurate Method for Titration of HIV, Lenti- and Retroviral Vectors

Quantification of retroviruses in cell culture supernatants and other biological preparations is required in a diverse spectrum of laboratories and applications. Methods based on antigen detection, such as p24 for HIV, or on genome detection are virus specific and sometimes suffer from a limited dyn...

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Detalhes bibliográficos
Main Authors: Vermeire, Jolien, Naessens, Evelien, Vanderstraeten, Hanne, Landi, Alessia, Iannucci, Veronica, Van Nuffel, Anouk, Taghon, Tom, Pizzato, Massimo, Verhasselt, Bruno
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3515444/
https://ncbi.nlm.nih.gov/pubmed/23227216
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0050859
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