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Temperature dependence of ssrA-tag mediated protein degradation

Building synthetic gene networks with highly transient dynamics requires rapid protein degradation. We show that the degradation conferred by two commonly used ssrA tags is highly temperature dependent. Synthetic gene networks are being used increasingly in real-world applications where they may be...

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Autori principali: Purcell, Oliver, Grierson, Claire S, Bernardo, Mario di, Savery, Nigel J
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3511798/
https://ncbi.nlm.nih.gov/pubmed/22824000
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1754-1611-6-10
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