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A simple protocol for the subcellular fractionation of skeletal muscle cells and tissue

BACKGROUND: We describe a method for subcellular fractionation of mouse skeletal muscle, myoblast and myotubes to obtain relatively pure fractions of nuclear, cytosolic and mitochondrial compartments. Fractionation allows the analysis of a protein of interest (or other cellular component) based on i...

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Main Authors: Dimauro, Ivan, Pearson, Timothy, Caporossi, Daniela, Jackson, Malcolm J
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2012
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3508861/
https://ncbi.nlm.nih.gov/pubmed/22994964
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-5-513
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