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A simple protocol for the subcellular fractionation of skeletal muscle cells and tissue
BACKGROUND: We describe a method for subcellular fractionation of mouse skeletal muscle, myoblast and myotubes to obtain relatively pure fractions of nuclear, cytosolic and mitochondrial compartments. Fractionation allows the analysis of a protein of interest (or other cellular component) based on i...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
BioMed Central
2012
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3508861/ https://ncbi.nlm.nih.gov/pubmed/22994964 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-5-513 |
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