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Homologous Alkalophilic and Acidophilic l-Arabinose Isomerases Reveal Region-Specific Contributions to the pH Dependence of Activity and Stability
To study the pH dependence of l-arabinose isomerase (AI) activity and stability, we compared homologous AIs with their chimeras. This study demonstrated that an ionizable amino acid near the catalytic site determines the optimal pH (pH(opt)) for activity, whereas the N-terminal surface R residues pl...
में बचाया:
| मुख्य लेखकों: | , , , , , |
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| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
American Society for Microbiology
2012
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3502938/ https://ncbi.nlm.nih.gov/pubmed/23001647 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02114-12 |
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