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FACS-based purification of Arabidopsis microspores, sperm cells and vegetative nuclei

BACKGROUND: The male germline in flowering plants differentiates by asymmetric division of haploid uninucleated microspores, giving rise to a vegetative cell enclosing a smaller generative cell, which eventually undergoes a second mitosis to originate two sperm cells. The vegetative cell and the spe...

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Main Authors: Borges, Filipe, Gardner, Rui, Lopes, Telma, Calarco, Joseph P, Boavida, Leonor C, Slotkin, R Keith, Martienssen, Robert A, Becker, Jörg D
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2012
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3502443/
https://ncbi.nlm.nih.gov/pubmed/23075219
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1746-4811-8-44
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