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Human lysosomes can be purified from diploid skin fibroblasts by free-flow electrophoresis.
Human diploid fibroblasts underwent lysis when resuspended in isotonic sucrose. After preparation of a granular fraction by differential centrifugation, lysosomes were almost completely purified by free-flow electrophoresis. Electron micrographs of the final fraction of fibroblast lysosomes (about 2...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1980
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350229/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6934540/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.10.6139 |
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