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Optimization of an Efficient Protein Extraction Protocol Compatible with Two-Dimensional Electrophoresis and Mass Spectrometry from Recalcitrant Phenolic Rich Roots of Chickpea (Cicer arietinum L.)

Two-dimensional electrophoresis and mass spectrometry are undoubtedly two essential tools popularly used in proteomic analyses. Utilization of these techniques however largely depends on efficient and optimized sample preparation, regarded as one of the most crucial steps for recovering maximum amou...

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Autori principali: Chatterjee, Moniya, Gupta, Sumanti, Bhar, Anirban, Das, Sampa
Natura: Artigo
Lingua:Inglês
Pubblicazione: Hindawi Publishing Corporation 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3502011/
https://ncbi.nlm.nih.gov/pubmed/23193474
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2012/536963
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