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Purification of myosin light chain kinase from bovine cardiac muscle.

Myosin light chain kinase was purified > 100,000-fold to apparent homogeneity with a yield of 10% from bovine cardiac muscle. Sodium dodecyl sulfate gels of the purified kinase showed one stained band corresponding to a Mr of 94,000. The enzyme was activated > 10-fold in the presence of Ca2+ (...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Wolf, H, Hofmann, F
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1980
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350169/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6934518/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.10.5852
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