Affinity chromatography in nonionic detergent solutions.

Anionic dye affinity chromatography is commonly unproductive in the presence of nonionic detergents used to extract particulate proteins. Using lactate dehydrogenase as a model protein, Cibacron blue F3GA as a model dye, and Triton X-100 as a model detergent, we find that the dye is encapsulated in...

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Detaylı Bibliyografya
Yayımlandı:Proc Natl Acad Sci U S A
Asıl Yazarlar: Robinson, J B, Strottmann, J M, Wick, D G, Stellwagen, E
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: National Academy of Sciences 1980
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350168/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6934517/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.10.5847
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