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In vitro synthesis of 1 alpha,25-dihydroxycholecalciferol and 24,25-dihydroxycholecalciferol by isolated calvarial cells.

The question of whether the skeleton metabolizes 25-hydroxycholecalciferol [25(OH)D3] to more-polar products was studied. Calvarial cells were dispersed from 16-day old chicken embryos by using collagenase and then grown in culture in serum-free medium. Confluent cell cultures were incubated with 7...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Turner, R T, Puzas, J E, Forte, M D, Lester, G E, Gray, T K, Howard, G A, Baylink, D J
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1980
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC350141/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6934505/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.10.5720
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