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Iminobiotin affinity columns and their application to retrieval of streptavidin.

A method is described for the retrieval of streptavidin from the culture broth of Streptomyces avidinii. The key step in this procedure is the adsorption of streptavidin from culture concentrates to an affinity column in which iminobiotin is attached to AH-Sepharose 4B. This column binds streptavbid...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Hofmann, K, Wood, S W, Brinton, C C, Montibeller, J A, Finn, F M
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1980
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349906/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6933515/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.8.4666
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