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DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.

We have developed a cell-free system for studying the synthesis of mRNA in mammalian cells. The system consists of a dialyzed and concentrated whole-cell extract derived from HeLa cells, small molecules and cofactors needed for transcription, and exogenously added DNA. Accurate transcription by RNA...

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Detalles Bibliográficos
Autores principales: Manley, J L, Fire, A, Cano, A, Sharp, P A, Gefter, M L
Formato: Artigo
Lenguaje:Inglês
Publicado: 1980
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC349725/
https://ncbi.nlm.nih.gov/pubmed/6933441
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