Identification of 5-methylcytosine in DNA fragments immobilized on nitrocellulose paper.
A method to identify 5-methylcytosine (m5Cyt) in DNA immobilized on nitrocellulose paper by using antibody against m5Cyt raised in rabbits is described. Immobilized restriction fragments of DNA are incubated first with purified antibody against m5Cyt and then with goat anti-rabbit IgG labeled with 1...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
1980
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349661/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6251470/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.6.3581 |
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