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The use of parthenotegenetic and IVF bovine blastocysts as a model for the creation of human embryonic stem cells under defined conditions

PURPOSES: Clinical application of human embryonic stem cells will be possible, when cell lines are created under xeno-free and defined conditions. We aimed to establish methodologies for parthenogenetic activation, culture to blastocyst and mechanical isolation of the inner cell mass (ICM) using bov...

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Bibliografiske detaljer
Main Authors: Ruggeri, R. R., Watanabe, Y., Meirelles, F., Bressan, F. F., Frantz, N., Bos-Mikich, A.
Format: Artigo
Sprog:Inglês
Udgivet: Springer US 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3492578/
https://ncbi.nlm.nih.gov/pubmed/23054358
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10815-012-9866-z
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