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Iterative plug-and-play methodology for constructing and modifying synthetic gene networks

We present a methodology for the design, construction, and modification of synthetic gene networks. This method emphasizes post-assembly modification of constructs based on network behavior, thus facilitating iterative design strategies and rapid tuning and repurposing of gene networks. The ease of...

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Autori principali: Litcofsky, Kevin D., Afeyan, Raffi B., Krom, Russell J., Khalil, Ahmad S., Collins, James J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3492501/
https://ncbi.nlm.nih.gov/pubmed/23042452
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2205
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