Localization of the puromycin binding site on the large ribosomal subunit of Escherichia coli by immunoelectron microscopy.

By using immunoelectron microscopy, we have localized the binding site on 50S Escherichia coli ribosomal subunits for puromycin, an antibiotic that interacts with the ribosomal peptidyltransferase center. This was achieved by affinity-labeling 50S subunits with N-bromoacetyl puromycin and treating t...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Lührmann, R, Bald, R, Stöffler-Meilicke, M, Stöffler, G
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1981
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349248/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7038674/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.12.7276
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