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Transmission electron microscopic method for gene mapping on polytene chromosomes by in situ hybridization.

A transmission electron microscope method for gene mapping by in situ hybridization to Drosophila polytene chromosomes has been developed. As electron-opaque labels, we use colloidal gold spheres having a diameter of 25 nm. The spheres are coated with a layer of protein to which Escherichia coli sin...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Wu, M, Davidson, N
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1981
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349194/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6947272/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.11.7059
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