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Improved crystallization of Escherichia coli ATP synthase catalytic complex (F(1)) by introducing a phosphomimetic mutation in subunit ∊

The bacterial ATP synthase (F(O)F(1)) of Escherichia coli has been the prominent model system for genetics, biochemical and more recently single-molecule studies on F-type ATP synthases. With 22 total polypeptide chains (total mass of ∼529 kDa), E. coli F(O)F(1) represents nature’s smallest rotary m...

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Detalhes bibliográficos
Main Authors: Roy, Ankoor, Hutcheon, Marcus L., Duncan, Thomas M., Cingolani, Gino
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3490465/
https://ncbi.nlm.nih.gov/pubmed/23027754
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112036718
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