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TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Several methods are available to manipulate bacterial chromosomes(1-3). Most of these protocols rely on the insertion of conditionally replicative plasmids (e.g. harboring pir-dependent or temperature-sensitive replicons(1,2)). These plasmids are integrated into bacterial chromosomes based on homolo...
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| 主要作者: | |
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| 格式: | Artigo |
| 語言: | Inglês |
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MyJove Corporation
2012
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3490321/ https://ncbi.nlm.nih.gov/pubmed/23093249 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3761 |
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