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TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

Several methods are available to manipulate bacterial chromosomes(1-3). Most of these protocols rely on the insertion of conditionally replicative plasmids (e.g. harboring pir-dependent or temperature-sensitive replicons(1,2)). These plasmids are integrated into bacterial chromosomes based on homolo...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Päätekijä: Blokesch, Melanie
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: MyJove Corporation 2012
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3490321/
https://ncbi.nlm.nih.gov/pubmed/23093249
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3761
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