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Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP

Multiphoton microscopy of intrinsic fluorescence and second harmonic generation (SHG) of whole mouse organs is made possible by optically clearing the organ before imaging.(1,2) However, for organs that contain fluorescent proteins such as GFP and YFP, optical clearing protocols that use methanol de...

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Hlavní autoři: Parra, Sonia G., Vesuna, Sam S., Murray, Teresa A., Levene, Michael J.
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3490273/
https://ncbi.nlm.nih.gov/pubmed/23023035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3848
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