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Storage conditions of intestinal microbiota matter in metagenomic analysis

BACKGROUND: The structure and function of human gut microbiota is currently inferred from metagenomic and metatranscriptomic analyses. Recovery of intact DNA and RNA is therefore a critical step in these studies. Here, we evaluated how different storage conditions of fecal samples affect the quality...

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Detalhes bibliográficos
Main Authors: Cardona, Silvia, Eck, Anat, Cassellas, Montserrat, Gallart, Milagros, Alastrue, Carmen, Dore, Joel, Azpiroz, Fernando, Roca, Joaquim, Guarner, Francisco, Manichanh, Chaysavanh
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3489833/
https://ncbi.nlm.nih.gov/pubmed/22846661
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-12-158
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