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Fluorometric assay for adenosine 3',5'-cyclic monophosphate-dependent protein kinase and phosphoprotein phosphatase activities.

A novel peptide substrate for adenosine 3',5'-cyclic monophosphate-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37), Leu-Arg-Arg-Trp-Ser-Leu-Gly, was synthesized. Phosphorylation of the peptide causes a 20% increase in the peptide fluorescence intensity at 358 nm. Values of Km...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Wright, D E, Noiman, E S, Chock, P B, Chau, V
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1981
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC348974/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6273844/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.10.6048
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