Optimization of de novo transcriptome assembly from high-throughput short read sequencing data improves functional annotation for non-model organisms

BACKGROUND: The k-mer hash length is a key factor affecting the output of de novo transcriptome assembly packages using de Bruijn graph algorithms. Assemblies constructed with varying single k-mer choices might result in the loss of unique contiguous sequences (contigs) and relevant biological infor...

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Main Authors: Haznedaroglu, Berat Z, Reeves, Darryl, Rismani-Yazdi, Hamid, Peccia, Jordan
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2012
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3489510/
https://ncbi.nlm.nih.gov/pubmed/22808927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-13-170
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