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Optimization of de novo transcriptome assembly from high-throughput short read sequencing data improves functional annotation for non-model organisms
BACKGROUND: The k-mer hash length is a key factor affecting the output of de novo transcriptome assembly packages using de Bruijn graph algorithms. Assemblies constructed with varying single k-mer choices might result in the loss of unique contiguous sequences (contigs) and relevant biological infor...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2012
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3489510/ https://ncbi.nlm.nih.gov/pubmed/22808927 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-13-170 |
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