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Directed evolution of bright mutants of an oxygen-independent flavin-binding fluorescent protein from Pseudomonas putida

BACKGROUND: Fluorescent reporter proteins have revolutionized our understanding of cellular bioprocesses by enabling live cell imaging with exquisite spatio-temporal resolution. Existing fluorescent proteins are predominantly based on the green fluorescent protein (GFP) and related analogs. However,...

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Detalhes bibliográficos
Main Authors: Mukherjee, Arnab, Weyant, Kevin B, Walker, Joshua, Schroeder, Charles M
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3488000/
https://ncbi.nlm.nih.gov/pubmed/23095243
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1754-1611-6-20
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