Expression, Secretion, and Glycosylation of the 45- and 47-kDa Glycoprotein of Mycobacterium tuberculosis in Streptomyces lividans

The gene encoding the 45/47 kDa glycoprotein (Rv1860) of Mycobacterium tuberculosis was expressed in Streptomyces lividans under its own promoter and under the thiostrepton-inducible Streptomyces promoter P(tipA). The recombinant protein was released into the culture medium and, like the native prot...

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Bibliographic Details
Main Authors: Lara, Martha, Servín-González, Luis, Singh, Mahavir, Moreno, Carlos, Cohen, Ingrid, Nimtz, Manfred, Espitia, Clara
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2004
Subjects:
Genetics and Molecular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC348798/
https://ncbi.nlm.nih.gov/pubmed/14766542
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.2.679-685.2004
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