Cloning of bacteriophage fd gene 2 and construction of a plasmid dependent on fd gene 2 protein.
Bacteriophage fd gene 2 was cloned in plasmid pBR325. Cells carrying the hybrid plasmid produce about 200 times more enzymatically active fd gene 2 protein than did cells infected with phage fd wild type, as measured by replication of phage fd replicative form I in vitro. Cloned gene 2 supports repl...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1981
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC348756/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6272301/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.9.5416 |
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