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Biosynthesis and periplasmic segregation of human proinsulin in Escherichia coli.
A plasmid containing human preproinsulin cDNA inserted into the endonuclease Pst I site of the ampicillinase gene of plasmid pBR322 was modified by excision of large portions of the ampicillinase-coding region to produce a variety of gene fusion combinations, many of which generated proteins detecta...
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| Main Authors: | , , , , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1981
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC348753/ https://ncbi.nlm.nih.gov/pubmed/7029534 |
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