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Online nanoscale ERLIC-MS outperforms RPLC-MS for shotgun proteomics in complex mixtures
We have explored the use of electrostatic repulsion hydrophilic interaction chromatography (ERLIC) as an alternative to the gold-standard in shotgun proteomics: reversed-phase (RP) LC for online ESI-MS/MS. Conditions for sample solubilization and initial gradient conditions were optimized to strike...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3487405/ https://ncbi.nlm.nih.gov/pubmed/22950739 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr300638n |
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