Isolation of the centromere-linked CDC10 gene by complementation in yeast.
A hybrid plasmid colony bank was constructed in Escherichia coli using the E. coli-Saccharomyces cerevisiae shuttle vector pLC544 and randomly sheared segments of yeast DNA. By transformation with a hybrid plasmid DNA pool from this collection and complementation of a temperature-sensitive cdc10 mut...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1980
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC348674/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6990421/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.4.2173 |
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