Bacteriophage lambda vehicle for the direct cloning of Escherichia coli promoter DNA sequences: feedback regulation of the rplJL-rpoBC operon.

A derivative of bacteriophage lambda, lambda 21, has been constructed and used for the cloning of Escherichia coli DNA fragments carrying promoters. Phage lambda 21 lacks the lac promoter operator and can accept DNA fragments up to 9.8 kilobases in size at a unique HindIII restriction endonuclease s...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Holowachuk, E W, Friesen, J D, Fiil, N P
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1980
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC348664/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6445564/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.4.2124
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