A Modified Technique for Culturing Primary Fetal Rat Cortical Neurons

The study explored a modified primary culture system for fetal rat cortical neurons. Day E18 embryos from pregnant Sprague Dawley rats were microdissected under a stereoscope. To minimize enzymatic damage to the cultured neurons, we applied a sequential digestion protocol using papain and Dnase I. T...

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Detalhes bibliográficos
Main Authors: Xu, Sui-Yi, Wu, Yong-Min, Ji, Zhong, Gao, Xiao-Ya, Pan, Su-Yue
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi Publishing Corporation 2012
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3486623/
https://ncbi.nlm.nih.gov/pubmed/23193366
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2012/803930
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