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In vitro synthesis of pre-proteins of vacuolar compartmented proteinase inhibitors that accumulate in leaves of wounded tomato plants

Two proteinase inhibitor proteins that are compartmented in leaf vacuoles (lysosomes) were synthesized in vitro. mRNA was isolated from 17-day-old expanding tomato leaves by extraction with chaotropic buffers followed by chromatography on oligo(dT)-cellulose and was translated with a rabbit reticulo...

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Sonraí Bibleagrafaíochta
Main Authors: Nelson, Christopher E., Ryan, Clarence A.
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1980
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC348632/
https://ncbi.nlm.nih.gov/pubmed/16592803
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