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Reconstitution of functional membrane-bound acetylcholine receptor from isolated Torpedo californica receptor protein and electroplax lipids.

Purified acetylcholine receptor and total lipids, both extracted from Torpedo californica electroplax, were utilized to reconstitute chemically excitable membrane vesicles. Reconstitution was achieved by dialysis of the extraction detergent, octyl beta-D-glucoside from protein/lipid incubation mixtu...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Gonzalez-Ros, J M, Paraschos, A, Martinez-Carrion, M
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1980
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC348594/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6246504/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.4.1796
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