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Insulin receptor synthesis and turnover in differentiating 3T3-L1 preadipocytes.

A density-shift method is described for analyzing insulin receptor synthesis and turnover in cultured cells labeled with "heavy" amino acids (2H, 13C, and 15N). Solubilized newly synthesized heavy and old "light" receptors are separated by isopycnic banding on CsCl gradients and...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Reed, B C, Lane, M D
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1980
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC348254/
https://ncbi.nlm.nih.gov/pubmed/6928620
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