Stereo-selective conversion of mandelonitrile to (R)-(−)-mandelic acid using immobilized cells of recombinant Escherichia coli

Immobilized cells of a recombinant Escherichia coli expressing nitrilase from Pseudomonas putida were used to catalyze the hydrolysis of mandelonitrile (2-hydroxy-2-phenylacetonitrile) to (R)-(−)-mandelic acid. The cells had been immobilized by entrapment in an alginate matrix. Conditions for the hy...

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Bibliografiske detaljer
Main Authors: Pawar, Sandip V., Meena, Vachan Singh, Kaushik, Shubhangi, Kamble, Ashwini, Kumar, Sandeep, Chisti, Yusuf, Banerjee, U. C.
Format: Artigo
Sprog:Inglês
Udgivet: Springer Berlin Heidelberg 2012
Fag:
Original Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3482447/
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-012-0058-4
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