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DNA of a circular minichromosome linearized by restriction enzymes or other reagents is resistant to further cleavage: an influence of chromatin topology on the accessibility of DNA

The accessibility of DNA in chromatin is an essential factor in regulating its activities. We studied the accessibility of the DNA in a ∼170 kb circular minichromosome to DNA-cleaving reagents using pulsed-field gel electrophoresis and fibre-fluorescence in situ hybridization on combed DNA molecules...

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Detalhes bibliográficos
Main Authors: Kumala, Sławomir, Hadj-Sahraoui, Yasmina, Rzeszowska-Wolny, Joanna, Hancock, Ronald
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3479189/
https://ncbi.nlm.nih.gov/pubmed/22848103
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks723
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