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Screening for catalytically active Type II restriction endonucleases using segregation-induced methylation deficiency
Type II restriction endonucleases (REases) are one of the basic tools of recombinant DNA technology. They also serve as models for elucidation of mechanisms for both site-specific DNA recognition and cleavage by proteins. However, isolation of catalytically active mutants from their libraries is cha...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3479162/ https://ncbi.nlm.nih.gov/pubmed/22753027 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks635 |
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