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Systematic Reverse Genetics of Transfer-DNA-Tagged Lines of Arabidopsis : Isolation of Mutations in the Cytochrome P450 Gene Superfamily
We have developed an efficient reverse-genetics protocol that uses expedient pooling and hybridization strategies to identify individual transfer-DNA insertion lines from a collection of 6000 independently transformed lines in as few as 36 polymerase chain reactions. We have used this protocol to sy...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society of Plant Physiologists
1998
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC34784/ https://ncbi.nlm.nih.gov/pubmed/9808718 |
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