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Construction and Validation of Lentiviral Vector Carrying Rat Neuronal Nitric Oxide Synthase In Vitro and In Vivo

In the present study, we developed a lentiviral vector with human cytomegalovirus promoter permitting high-level of nNOS expression. Neuronal cell line NG108 was used as an in vitro model to check the validity of gene transfer. The cells were infected with lenti-EGFP or lenti-nNOS particles for 24h....

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Detalhes bibliográficos
Main Authors: Leite, Laura H. R., Sharma, Neeru M., Bafna, Sangeeta, Zheng, Hong, Coimbra, Cândido C., Patel, Kaushik P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3476942/
https://ncbi.nlm.nih.gov/pubmed/22921486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jneumeth.2012.08.012
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