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A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Recombinant protein expression in bacteria, typically E. coli, has been the most successful strategy for milligram quantity expression of proteins. However, prokaryotic hosts are often not as appropriate for expression of human, viral or eukaryotic proteins due to toxicity of the foreign macromolecu...

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Detalhes bibliográficos
Main Authors: Aydin, Halil, Azimi, Farshad C., Cook, Jonathan D., Lee, Jeffrey E.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3476395/
https://ncbi.nlm.nih.gov/pubmed/22872008
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/4041
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