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Genetic and biochemical analysis of gonococcal IgA1 protease: cloning in Escherichia coli and construction of mutants of gonococci that fail to produce the activity.
The biological significance of bacterial extracellular proteases that specifically cleave human IgA1 is unknown. We have prepared a gene bank of gonococcal chromosomal DNA in Escherichia coli K-12 using a cosmid cloning system. Among these clones, we have identified and characterized an E. coli stra...
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Hauptverfasser: | , , |
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Format: | Artigo |
Sprache: | Inglês |
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1982
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Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC347453/ https://ncbi.nlm.nih.gov/pubmed/6818556 |
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